Enhanced chemiluminescence biosensor for the determination of phenolic compounds and hydrogen peroxide

The enhanced chemiluminescence (CL) reaction of the luminol-H2O2-horseradis h peroxidase (HRP) system with immobilized HRP using microencapsulation in a sol-gel matrix has been used to develop a biosensor for p-iodophenol, p-c oumaric acid, 2-naphthol and hydrogen peroxide. The detection limits obtain ed for p-iodophenol, p-coumaric acid and 2-naphthol were 0.83 muM, 15 and 4 8 nM, respectively. Direct enzyme immobilization onto the end of the optica l fibre permits the construction of a remote enhanced CL biosensor. This re mote biosensor has been applied to hydrogen peroxide assay (detection limit 52.2 muM and relative standard deviation 4.7% for n = 4) and the results h ave been compared with the biosensor developed in cuvette. (C) 2001 Elsevie r Science B.V. All rights reserved.