SPECIFIC INDUCTION OF INTERLEUKIN-4-PRODUCING CELLS IN RESPONSE TO IN-VITRO ALLERGEN STIMULATION IN ATOPIC INDIVIDUALS

Background and Objective CD4(+) cells can be divided into two major su bsets, T helper (TH)(1) and TH2 cells. Interleukin-4 (IL-4) is produce d by TH2 cells and induces switching of immunoglobulin (Ig) M/IgG to I gE. Interferon-gamma (IFN gamma) produced by TH1 cells counteracts the IgE-promoting effects of IL-4. In this study we wanted to investigate whether the number of IL-4-producing cells could be a direct measurem ent of allergen exposure in vitro, and whether this was correlated to the elevated serum IgE-levels seen in atopic persons. Methods We compa red the number of IL-4- and IFN gamma-producing cells using an enzyme- linked immunospot assay (ELISPOT) in response to allergens from birch and rat in peripheral mononuclear cells from atopic and healthy indivi duals. Results In the two sensitized groups there was an increase in t he number of IL-4-producing cells in response to the specific allergen which was not seen in the healthy group (1/20 000 cells and 1/200 000 cells, respectively, P < 0.001 for birch). In criss-cross experiments where birch-sensitized individuals were stimulated with cat allergen, no IL-4-producing cells were seen, indicating a high degree of specif icity. in individual subjects, the elevated numbers of IL-4-producing cells were significantly correlated with their allergen-specific serum IgE levels, When allergen was combined with a suboptimal dose of PHA, there was a synergistic increase in the number of allergen-induced IL -4-producing cells (1/4 000 cells) in the atopic donors, which was not seen with the number of IFN gamma-producing cells. Conclusions Allerg en-specific IL-I producing cells in a peripheral blood mononuclear cel l (PBMC) culture can be detected by ELISPOT and the response can syner gistically be enhanced by suboptimal concentrations of PHA.