Comparison of manganese superoxide dismutase precursor induction ability in human hepatoma cells with or without hepatitis B virus DNA insertion

In a previous study (Hajnicka ct nl.. Acta virol. 38. 55-57 (1994)): we des cribed synthesis of a 23 K protein in high amounts in the PLC/PRF/5 human h epatoma cell line after stimulation with sera of patients suffering from li ver cirrhosis. In this study we identified this protein as manganense super oxide dismutase (Mn-SOD). When PLC/PRF/5 cells stimulated by various cytoki nes (interleukin-1 alpha (IL-1 alpha), IL-1 beta, tumor necrosis factor -al pha (TNF-alpha),TNF-beta, IL-6, tumor growth factor-alpha (TGF-alpha), TGF- beta, and interferon-gamma (IFN-gamma) were compared, the most effective wa s IL-1, followed by TNF-alpha and IL-6, Other cytokines had no effect on th e stimulation of Mn-SOD. IL-1 alpha was selected for stimulation of Mn-SOD production in four human hepatoma cell lines (PLC/PRF/5,Hep-3B, Hep-G2 and Sk-Hep 1). Maximum Mn-SOD production occured in PLC/PRF/5 cells. In other c ell lines, Mn-SOD production was lower. reaching 35.7% and 31.5% in Hep-3B and Sk-Hep-1 cells, respectively, while it was only 4.3% in Hep-G2 cells.