A. Joly et al., INFLUENCE OF LIPIDS ON DIMETHYLAMINE FORMATION IN MODEL SYSTEMS OF HAKE (MERLUCCIUS-MERLUCCIUS) KIDNEY DURING FROZEN STORAGE, ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG A-FOOD RESEARCH AND TECHNOLOGY, 205(1), 1997, pp. 14-18
The role of non-oxidized and oxidized lipids in fish muscle proteins d
uring frozen storage is a matter of controversy: some studies have sho
wn that they have a detrimental effect, whereas others have shown a pr
otective effect through a reduction in the amount of formaldehyde (FA)
and dimethylamine (DMA) formed from trimethylamine N-oxide (TMAO) dur
ing frozen storage. A model system based on an extract of hake kidney
as a source of trimethylamine N-oxide demethylase (TMAO-ase) and the s
ubstrate TMAO was prepared to study the effects of nonoxidized lipids
and oxidized lipids with increasing peroxide values (POV) and of a cry
oprotectant (glycerol) on TMAO-ase activity during frozen storage, usi
ng response surface methodology (RSM). At low POV, the oxidized lipids
increased DMA formation, but at oxidation levels equal to or higher t
han 500 meq kg(-1) they reduced the amount of DMA produced. This behav
iour corroborates the hypothesis that oxidizing/reducing conditions st
rongly influence the capacity of the enzyme to catalyse the reaction.
However, non-oxidized lipids were found to have a very weak inhibitory
effect. Glycerol had a significant inhibitory effect on DMA formation
, greater than that of oxidized lipids in the present enzymatic system
.