Endothelin-1 (ET-1) has been shown to be mitogenic for endothelial and seve
ral tumor cells through an autocrine mechanism. In this study we evaluated
whether the tumorigenic KS IMM cell line deriving from Kaposi's sarcoma (KS
), a highly angiogenic tumor, is susceptible to ET-1 mitogenic activity. By
reverse transcriptase-polymerase chain reaction, we detected ET-1 mRNA exp
ression and both ETA receptor (ETAR) and ETBR mRNA transcripts in the KS IM
M cells. High concentrations of ET-1 are released from the KS IMM cells and
competition-binding studies demonstrated that these cells also express fun
ctional ETAR and ETBR with high affinity for ET-1 and ET-1/ ET-3, respectiv
ely. Expression of ET-1 and cognate receptors could be detected by immunohi
stochemical method in vitro, in KS IMM xenograft, and in tissue sections of
a human KS lesion. Furthermore ET-1 induces a marked and dose-dependent in
crease in [H-3]thymidine incorporation comparable to that elicited by vascu
lar endothelial growth factor. Addition of both selective ETBR antagonist (
BQ 788) and ETAR antagonist (BQ 123), completely blocked ET-1-induced mitog
enic response and reduced the basal growth rate of unstimulated cells, sugg
esting that both receptors mediated the proliferative signal. Such findings
demonstrate that ET-1 participates on KS pathogenesis acting as an autocri
ne growth factor and that ET-1 receptor antagonists may thus be novel candi
dates for therapeutic intervention.