Circulating monocytes are not the source of elevations in plasma IL-6 and TNF-alpha levels after prolonged running

Citation
Rl. Starkie et al., Circulating monocytes are not the source of elevations in plasma IL-6 and TNF-alpha levels after prolonged running, AM J P-CELL, 280(4), 2001, pp. C769-C774
Citations number
29
Categorie Soggetti
Cell & Developmental Biology
Journal title
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
ISSN journal
03636143 → ACNP
Volume
280
Issue
4
Year of publication
2001
Pages
C769 - C774
Database
ISI
SICI code
0363-6143(200104)280:4<C769:CMANTS>2.0.ZU;2-R
Abstract
The present study was undertaken to examine the effect of prolonged running on monocyte intracellular cytokine production and plasma cytokine concentr ation. Blood samples were collected 1 h before, immediately after, 2 h afte r, and 24 h after a competitive marathon run. There was no change in the nu mber of cells spontaneously producing tumor necrosis factor (TNF)-alpha; ho wever, there was a decrease in the number of cells producing interleukin (I L)-1 alpha and IL-6 (P < 0.01) postexercise. In contrast, there was an incr ease in the number of monocytes that responded to lipopolysaccharide stimul ation by producing IL-1<alpha>, TNF-alpha, and IL-6 (P < 0.01) immediately and 2 h postexercise; however, these cells contained less cytokine (P < 0.0 5). Plasma IL-6, TNF-alpha, epinephrine, norepinephrine, and cortisol conce ntrations were markedly increased (P < 0.01) postexercise. These data demon strate that circulating monocytes are not the source of elevated levels of plasma IL-6 and TNF-<alpha> after prolonged running. In addition, it is lik ely that stress hormones result in a decrease in the amount of cytokine pro duced by LPS-stimulated cells postexercise.