Further development of a recombinant feline herpesvirus type 1 expressing the Gag protein of feline immunodeficiency virus

Authors
Sato, E Miyazawa, T Nishimura, Y Ikeda, Y Kawakami, K Mochizuki, M Yokoyama, N Maeda, K Fujita, K Kohmoto, M Takahashi, E Mikami, T
Citation
E. Sato et al., Further development of a recombinant feline herpesvirus type 1 expressing the Gag protein of feline immunodeficiency virus, ARCH VIROL, 146(2), 2001, pp. 379-387
Citations number
28
Categorie Soggetti
Microbiology
Journal title
ARCHIVES OF VIROLOGY
ISSN journal
03048608 → ACNP
Volume
146
Issue
2
Year of publication
2001
Pages
379 - 387
Database
ISI
SICI code
0304-8608(2001)146:2<379:FDOARF>2.0.ZU;2-S
Abstract
We have previously reported the construction of a recombinant feline herpes virus type 1 (FHV-1), designated C7301ddlTK-gag, expressing the Gag precurs or protein of feline immunodeficiency virus (FIV). In this study, we report the construction of a further recombinant FHV-1 (ddlTK(gBp)-gag) which car ries an FHV-1 gB promoter sequence upstream of the FIV gag gene of C7301ddl TK-gag. Strong expression of the FIV Gag protein by dnlTK(gBp)-gag was conf irmed by immunoblot analyses and enzyme-linked immunosorbent assays. Althou gh C7301ddlTK-gag and ddlTK(gBp)-gag failed to induce anti-FIV Gag antibodi es in cats, we confirmed the infectivity and stability of these recombinant s in cats.