A facile approach to immobilize protein for biosensor: self-assembled supported bilayer lipid membranes on glassy carbon electrode

A kind of solid substrate, glassy carbon (GC) electrode. was selected to su pport self-assembled lipid layer membranes. On the surface of GC electrode. we made layers of dimyristoylphosphatidylcholine (DMPG, a kind of lipid). From electrochemical impedance experiments. we demonstrated that the lipid layers on the GC electrode were bilayer lipid membranes. We immobilized hor seradish peroxidase (HRP) into the supported bilayer lipid membranes (s-BLM ) to develop a kind of mediator-free biosensor for H2O2. The biosensor exhi bited fine electrochemical response, stability and reproducibility due to t he presence of the s-BLM. As a model of biological membrane, s-BLM could su pply a biological environment for enzyme and maintain its activity. So s-BL M is an ideal choice to immobilize enzyme for constructing the mediator-fre e biosensor based on GC electrode. (C) 2001 Elsevier Science B.V. All right s reserved.