A surface plasmon resonance array biosensor based on spectroscopic imaging

We have developed a multi-element transduction system which combines conven tional SPR spectroscopy with one-dimensional SPR microscopy to create an ef fective platform for monitoring binding events on macro- or micro-patterned receptor arrays created on disposable sensor chips. This creates an effect ive platform for monitoring simultaneous binding events on each of the regi ons patterned with the receptors. This system has been specifically designe d with commercially available components to allow relatively easy duplicati on. Furthermore. this system can use a proven, simple method to compensate for changes in the bulk index of refraction of the solution containing the analytes due to changes in temperature or solute concentration with simple modifications to the sensor chips alone. Preliminary results demonstrate ho w this system can be used to monitor several independent biospecific bindin g events simultaneously. (C) 2001 Elsevier Science B.V. All rights reserved .