DETECTION OF DIFFERENTIALLY EXPRESSED GENES IN HUMAN BLADDER-CANCER CELLS USING ARBITRARILY PRIMED PCR OF RNA

The aim of the present study was to detect differentially expressed ge nes in human bladder cancer cell lines using a non-radioactive RNA fin gerprinting technique (arbitrarily primed polymerase chain reaction of RNA, RAP-PCR). The two clonal urothelial cancer cell lines, RT4 and J 82, show different growth kinetics upon stimulation with EGF. By RAP-P CR we detected changes in band patterns for J82 cells treated with EGF but not for RT4 cells. Polymorphic fragments were further characteriz ed and sequences from two of these gave a perfect match to the coding sequence of the human tropomyosin gene TM30(pl) and the human MAC30 ge ne, respectively. In accordance with the results of RAP-PCR downregula tion in EGF-stimulated J82 cells could be demonstrated by reverse tran scription PCR.