CRYSTALLIZATION AND PRELIMINARY-X-RAY CRYSTALLOGRAPHIC ANALYSIS OF THE ELECTRON-TRANSFERRING FLAVOPROTEIN FROM MEGASPHAERA-ELSDENII

Authors
SHARKEY CT WALSH MA MAYHEW SG HIGGINS TM
Citation
Ct. Sharkey et al., CRYSTALLIZATION AND PRELIMINARY-X-RAY CRYSTALLOGRAPHIC ANALYSIS OF THE ELECTRON-TRANSFERRING FLAVOPROTEIN FROM MEGASPHAERA-ELSDENII, Acta crystallographica. Section D, Biological crystallography, 53, 1997, pp. 461-463
Citations number
19
Categorie Soggetti
Crystallography,"Biochemical Research Methods",Biology
Journal title
Acta crystallographica. Section D, Biological crystallography → ACNP
ISSN journal
09074449
Volume
53
Year of publication
1997
Part
4
Pages
461 - 463
Database
ISI
SICI code
0907-4449(1997)53:<461:CAPCAO>2.0.ZU;2-U
Abstract
Electron-transferring flavoprotein from the rumen bacterium Megasphaer a elsdenii is a heterodimer (M-r = 75 kDa) containing FAD as cofactor and functioning solely to mediate electron transfer between the prosth etic groups of other proteins. The enzyme was crystallized by the hang ing-drop vapour-diffusion method using polyethylene glycol 4000 as pre cipitant. The crystals obtained belong to the space group P2(1)2(1)2(1 ) with unit-cell dimensions of a = 58.75, b = 61.77 and c = 122.27 Ang strom. Interestingly the crystals exhibit a low solvent content. Cryst als diffracted to beyond 2.5 Angstrom using synchrotron radiation.