PURIFICATION, CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF THE M.BSECI DNA METHYLTRANSFERASE FROM BACILLUS-STEAROTHERMOPHILUS

Authors
ATHANASIADIS A PAPANIKOLAU Y RINA M PAPADOVASILAKI M DAUTER Z PETRATOS K BOURIOTIS V KOKKINIDIS M
Citation
A. Athanasiadis et al., PURIFICATION, CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF THE M.BSECI DNA METHYLTRANSFERASE FROM BACILLUS-STEAROTHERMOPHILUS, Acta crystallographica. Section D, Biological crystallography, 53, 1997, pp. 477-479
Citations number
26
Categorie Soggetti
Crystallography,"Biochemical Research Methods",Biology
Journal title
Acta crystallographica. Section D, Biological crystallography → ACNP
ISSN journal
09074449
Volume
53
Year of publication
1997
Part
4
Pages
477 - 479
Database
ISI
SICI code
0907-4449(1997)53:<477:PCAPAO>2.0.ZU;2-F
Abstract
The DNA methyltransferase M.BseC1 from B. stearothermophilus methylate s the N6 atom of the 3' adenine in the sequence 5'-ATCGAT-3'. The 579- residue protein has been isolated and crystallized using seeding and m icrodialysis techniques. The crystals are monoclinic, space group P2(1 ) with cell dimensions a = 53.7, b = 85.7, c = 1518 Angstrom and beta = 95.1 degrees, two molecules in the asymmetric unit and diffract to a t least 2.5 Angstrom resolution.