Duration of sample storage dramatically alters expression of the human immunodeficiency virus coreceptors CXCR4 and CCR5

Authors
Shalekoff, S Tiemessen, CT
Citation
S. Shalekoff et Ct. Tiemessen, Duration of sample storage dramatically alters expression of the human immunodeficiency virus coreceptors CXCR4 and CCR5, CL DIAG LAB, 8(2), 2001, pp. 432-436
Citations number
14
Categorie Soggetti
Immunology
Journal title
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
ISSN journal
1071412X → ACNP
Volume
8
Issue
2
Year of publication
2001
Pages
432 - 436
Database
ISI
SICI code
1071-412X(200103)8:2<432:DOSSDA>2.0.ZU;2-T
Abstract
Expression of the chemokine receptors CXCR4 and CCR5 was monitored using ED TA-anticoagulated whole blood held for different time periods prior to fluo rescent-antibody staining. When left overnight CXCR4 expression on leukocyt es was substantially increased, whereas CCR5 expression was reduced. The re sults were similar when heparin and acid-citrate-dextrose were used as anti coagulants.