Jd. Bhawalkar et al., NONDESTRUCTIVE EVALUATION OF POLYMERIC PAINTS AND COATINGS USING 2-PHOTON LASER-SCANNING CONFOCAL MICROSCOPY, Polymer, 38(17), 1997, pp. 4551-4555
A 120 mu m thick tri-layer polymer paint on an aluminium substrate was
imaged in three-dimensions using multichannel two-photon confocal las
er scanning microscopy. Each layer is doped with a different fluoropho
re so that the fluorescence emission from the layers can be separated
into different channels. Use of i.r. excitation light allows deeper pe
netration in the polymer layers. The image reveals fine details within
each layer, as well as information about the interfaces between layer
s. The substrate features are also visible with excellent resolution i
n negative contrast. This technique provides a nondestructive method t
o probe thick multilayer polymer materials. (C) 1997 Elsevier Science
Ltd.