New mutants of Neurospora crassa having the ufa phenotype have been is
olated. Two of these mutants, like previously identified ufa mutants,
require an unsaturated fatty acid for growth and are almost completely
blocked in the de novo synthesis of unsaturated fatty acids. The new
mutations map to a different chromosomal location than previously char
acterized ufa mutations. This implies that at least one additional gen
etic locus controls the synthesis of unsaturated fatty acids in Neuros
pora.