Phylogenetic analysis of infectious salmon anaemia virus isolates from Norway, Canada and Scotland

Citation
B. Krossoy et al., Phylogenetic analysis of infectious salmon anaemia virus isolates from Norway, Canada and Scotland, DIS AQU ORG, 44(1), 2001, pp. 1-6
Citations number
32
Categorie Soggetti
Aquatic Sciences
Journal title
DISEASES OF AQUATIC ORGANISMS
ISSN journal
01775103 → ACNP
Volume
44
Issue
1
Year of publication
2001
Pages
1 - 6
Database
ISI
SICI code
0177-5103(20010126)44:1<1:PAOISA>2.0.ZU;2-0
Abstract
The sequences of gene segments 2 and 8 from 10 different isolates of infect ious salmon anaemia Virus (ISAV) sampled in Norway, Canada and Scotland bet ween 1987 and 1999 were determined and compared. Pairwise comparisons revea led a high degree of homology between the European isolates, with identitie s of 98 to 100% for both genes examined. The Canadian isolate showed identi ties of 84 and 87 to 88 % with the European isolates for the nucleotide seq uence of segments 2 and 8, respectively. Phylogenetic analyses were perform ed to establish the interrelationship between the European virus isolates. The evolutionary rate based on 4 Norwegian isolates clustered together in t he analysis of segment 2 was calculated to be 0.96 x 10(-3) nucleotides sit e(-1) yr(-1). On the basis of this mutation rate it was estimated that the Norwegian Glesvaer 90 and Canadian Bay of Fundy 97 isolates diverged around 1900, which coincides with transportation of salmonids between Europe and North America starting in the late nineteenth century.