Identification of fish-parasitic Myxobolus (Myxosporea) species using a combined PCR-RFLP method

Authors
Eszterbauer, E Benko, M Dan, A Molnar, K
Citation
E. Eszterbauer et al., Identification of fish-parasitic Myxobolus (Myxosporea) species using a combined PCR-RFLP method, DIS AQU ORG, 44(1), 2001, pp. 35-39
Citations number
28
Categorie Soggetti
Aquatic Sciences
Journal title
DISEASES OF AQUATIC ORGANISMS
ISSN journal
01775103 → ACNP
Volume
44
Issue
1
Year of publication
2001
Pages
35 - 39
Database
ISI
SICI code
0177-5103(20010126)44:1<35:IOFM(S>2.0.ZU;2-6
Abstract
Polymerase chain reaction (PCR) with primers specific for the family Myxobo lidae was used to amplify a part of the 18S ribosomal RNA gene of Myxobolus species. The length of the amplified fragments was approximately 1600 base pairs. Six Myxobolus species identified on the basis of morphological feat ures were compared using a combined PCR-RFLP method. The cleavage patterns generated by 2 frequent cutter restriction enzymes (HinfI and MspI) were su itable for the differentiation of the examined Myxobolus species.