Tissue distribution and temperature-dependence of Anguilla anguilla L. EROD activity following exposure to model inducers and relationship with plasma cortisol, lactate and glucose levels

Anguilla anguilla L. ethoxyresorufin-O-deethylase (EROD) elevation by 2.7 m uM beta -naphthoflavone (BNF) 3 days water exposure, or 4 mg/kg ip exposure , was studied in four different organs - liver, kidney, gills, and intestin e. The results demonstrated a significant increase in liver EROD activity f or the two previous conditions, whereas kidney EROD activity only increased during the intraperitoneal exposure. A. anguilla was also exposed during 8 , 16, and 24 h to water contaminated with 2.7 muM BNF or benzo[a]pyrene (Ba P) at 20 degreesC and 25 degreesC. Both compounds significantly increased l iver EROD activity from 8 up to 24 h. There was no significant difference i n liver EROD activity elevation by both compounds, either at 20 degreesC or 25 degreesC. Liver EROD activity was demonstrated to be one of the first w arning systems concerning the presence of polycyclic aromatic hydrocarbons (PAHs) in water. A. anguilla 3 h exposure to diesel oil water-soluble fract ion (DWSF) significantly increased plasma cortisol and significantly decrea sed plasma lactate. A prolonged exposure beyond 3 h, i.e. 4 h, 2, 3, 4, and 6 days to the previous conditions demonstrated a significant liver EROD ac tivity elevation from Day 2 up to 6, and a significant increase in erythroc ytic nuclear abnormalities (ENA) at Day 6. (C) 2001 Elsevier Science Ltd. A ll rights reserved.