Micropropagation of Cymbidium ensifolium var. misericors through callus-derived rhizomes

The effects were studied of light intensity, culture method and cytokinins on plant formation from callus-derived rhizomes of Cymbidium ensifolium var . misericors. The results demonstrated that one piece of rhizome produced s even shoot buds in 45 d when cultured in 1/2 MS basal liquid medium supplem ented with 0.17 muM N-6-(2-isopentenyl) adenine: 0.17 muM thidiazuron, 33 m uM 6-aminopurine adenine and 1.5 muM naphthaleneacetic acid under 10 mu mol m(-2) s(-1) artificial light and agitated at 60 rpm on a rotary shaker. Th ese shoot-cat plantlets in 5 mo. when transferred to the same Gelrite-gel b asal medium supplemented with 50 g 1(-1) banana pulp. Plantlets were acclim ated and grew well when potted in the greenhouse.