Jp. Henderson et al., Production of brominating intermediates by myeloperoxidase - A transhalogenation pathway for generating mutagenic nucleobases during inflammation, J BIOL CHEM, 276(11), 2001, pp. 7867-7875
The existence of interhalogen compounds was proposed more than a century ag
o, but no biological roles have been attributed to these highly oxidizing i
ntermediates. In this study, we determined whether the peroxidases of white
blood cells can generate the interhalogen gas bromine chloride (BrCl), Mye
loperoxidase, the heme enzyme secreted by activated neutrophils and monocyt
es, uses H2O2 and Cl- to produce HOCl, a chlorinating intermediate. In cont
rast, eosinophil peroxidase preferentially converts Br- to HOBr, Remarkably
, both myeloperoxidase and eosinophil peroxidase were able to brominate deo
xycytidine, a nucleoside, and uracil, a nucleo,ase, at plasma concentration
s of Br- (100 muM) and Cl- (100 mM). The two enzymes used different reactio
n pathways, however. When HOCl brominated deoxycytidine, the reaction requi
red Br- and was inhibited by taurine. In contrast, bromination by HOBr was
independent of Br- and unaffected by taurine, Moreover, taurine inhibited 5
-bromodeoxycytidine production by the myeloperoxidase-H2O2-Cl-- Br- system
but not by the eosinophil peroxidase-H2O2-Cl--Br- system, indicating that b
romination by myeloperoxidase involves the initial production of HOCl. Both
HOCl-Br- and the myeloperoxidase-H2O2-Cl--Br- system generated a gas that
converted cyclohexene into 1-bromo-2-chlorocyclohexane, implicating BrCl in
the reaction. Moreover, human neutrophils used myeloperoxidase, H2O2, and
Br- to brominate deoxycytidine by a taurine-sensitive pathway, suggesting t
hat transhalogenation reactions may be physiologically relevant. 5-Bromoura
cil incorporated into nuclear DNA is a well known mutagen. Our observations
therefore raise the possibility that transhalogenation reactions initiated
by phagocytes provide one pathway for mutagenesis and cytotoxicity at site
s of inflammation.