Identification of the bile acid-binding site of the ileal lipid-binding protein by photoaffinity labeling, matrix-assisted laser desorption ionization-mass spectrometry, and NMR structure

The ileal lipid-binding protein (ILBP) is the only physiologically relevant bile acid-binding protein in the cytosol of ileocytes, To identify the bil e acid-binding site(s) of ILBP, recombinant rabbit ILBP photolabeled with 3 -azi- and 7-azi-derivatives of cholyltaurine was analyzed by a combination of enzymatic fragmentation, gel electrophoresis, and matrix-assisted laser desorption ionization (MALDI)-mass spectrometry, The attachment site of the 3-position of cholyltaurine was localized to the amino acid triplet His(10 0)-Thr(101)-Ser(102) using the photoreactive 3,3-azo-derivative of cholylta urine, With the corresponding 7,7-azo-derivative, the attachment point of t he 7-position could be localized to the C-terminal part (position 112-128) as well as to the N-terminal part suggesting more than one binding site for bile acids. By chemical modification and MMR structure of ILBP, arginine r esidue 122 was identified as the probable contact point for the negatively charged side chain of cholyltaurine, Consequently, bile acids bind to ILBP with the steroid nucleus deep inside the protein cavity and the negatively charged side chain near the entry portal, The combination of photoaffinity labeling, enzymatic fragmentation, MALDI-mass spectrometry, and NMR structu re was successfully used to determine the topology of bile acid binding to ILBP.