Activation of the murine EP3 receptor for PGE(2) inhibits cAMP production and promotes platelet aggregation

The importance of arachidonic acid metabolites (termed eicosanoids), partic ularly those derived from the COX-1 and COX-2 pathways (termed prostanoids) , in platelet homeostasis has long been recognized, Thromboxane is a potent agonist, whereas prostacyclin is an inhibitor of platelet aggregation. In contrast, the effect of prostaglandin E-2 (PGE(2)) on platelet aggregation varies significantly depending on its concentration. Low concentrations of PGE(2) enhance platelet aggregation, whereas high PGE(2) levels inhibit agg regation. The mechanism for this dual action of PGE(2) is not clear. This s tudy shows that among the four PGE(2) receptors (EP1-EP4), activation of EP 3 is sufficient to mediate the proaggregatory actions of low PGE(2) concent ration. In contrast, the prostacyclin receptor (IP) mediates the inhibitory effect of higher PGE(2) concentrations. Furthermore, the relative activati on of these two receptors, EP3 and IP, regulates the intracellular level of cAMP and in this way conditions the response of the platelet to aggregatin g agents. Consistent with these findings, loss of the EP3 receptor in a mod el of venous inflammation protects against formation of intravascular clots . Our results suggest that local production of PGE(2) during an inflammator y process can modulate ensuing platelet responses.