Microarray profiling of gene expression in aging and its alteration by caloric restriction in mice

An active research area in biological gerontology concerns the mechanisms b y which caloric restriction (CR) retards the aging process in laboratory ro dents. We used high density oligonucleotide arrays representing 6347 genes to determine the gene expression profile of the aging process in gastrocnem ius muscle of male C57BL/6 mice. Aging resulted in a differential gene expr ession pattern indicative of a marked stress response and lower expression of metabolic and biosynthetic genes. Most alterations were completely or pa rtially prevented by CR. Transcriptional patterns of muscle from calorie-re stricted animals suggest that CR retards the aging process by causing a met abolic shift toward increased protein turnover and decreased macromolecular damage. The use of high density oligonucleotide microarrays provides a new tool to measure biological age on a tissue-specific basis and to evaluate at the molecular level the efficacy of nutritional interventions designed t o retard the aging process.