The influence of nicotine on the bone loss rate in ligature-induced periodontitis. A histometric study in rats

Background: The present study investigated the possible influence of nicoti ne on the bone loss rate in the furcation region due to ligature-induced pe riodontitis in rats. Methods: Twenty adult male Wistar rats were included. After anesthesia, the tooth was randomly assigned to receive the cotton ligature in the sulcular area, while the contralateral tooth was left unligated. The animals were r andomly assigned to one of the following treatments, including daily intrap eritoneal injections: group A, 2 mul/g body weight of saline solution; grou p B, 2 mul/g body weight of a nicotine solution with 0.13 mul of nicotine/m l of saline solution; group C, 2 mul/g body weight of a nicotine solution w ith 0.19 mul of nicotine/ml of saline solution; and group D, 2 mul/g body w eight of a nicotine solution with 0.26 mul of nicotine/ml of saline solutio n. Thirty days later, the animals were sacrificed and the specimens routine ly processed for serial decalcified sections. Results: Intergroup analysis revealed greater bone loss in the ligated teet h of group B (1.01 +/- 0.61 mm(2)), group C (1.14 +/- 0.72 mm(2)), and grou p D (1.36 +/- 0.60 mm(2)) when compared with group A (0.64 +/- 0.62 mm(2)) (P <0.01). However, no statistically significant differences in bone loss w ere found among groups B, C, and D. In addition, no bone loss was observed for unligated teeth (P >0.01). Conclusions: Within the limits of the present study, nicotine enhanced the effects of the local components of periodontal disease in a non-dose-depend ent way; nevertheless, the administration of nicotine did not produce perio dontal bone loss by itself.