The mouthwash: A non-invasive sampling method to study cytokine gene polymorphisms

Citation
Ml. Laine et al., The mouthwash: A non-invasive sampling method to study cytokine gene polymorphisms, J PERIODONT, 71(8), 2000, pp. 1315-1318
Citations number
22
Categorie Soggetti
Dentistry/Oral Surgery & Medicine","da verificare
Journal title
JOURNAL OF PERIODONTOLOGY
ISSN journal
00223492 → ACNP
Volume
71
Issue
8
Year of publication
2000
Pages
1315 - 1318
Database
ISI
SICI code
0022-3492(200008)71:8<1315:TMANSM>2.0.ZU;2-S
Abstract
Background: We describe a simple, non-invasive mouthwash sampling method fo r rapid DNA isolation to detect cytokine gene polymorphisms. In the present paper, interleukin-1 beta (IL-1B) and interleukin-1 receptor antagonist (I L-1RN) gene polymorphisms were studied. Methods: Two mouthwash samples and blood samples were collected from 11 hea lthy individuals. The second mouthwash sample was stored for 7 days at room temperature. Polymerase chain reaction amplification was used to identify a bi-allelic polymorphism at position +3953 in the IL-1B gene and a variabl e number of tandem repeats (VNTR) polymorphism in the IL-1RN gene. Results: Our results show that the typing of these cytokine gene polymorphi sms using DNA isolated from mouthwash samples did not differ from those obt ained by a phenol/chloroform isolation method from EDTA anti-coagulated blo od. Moreover, reliable results from mouthwash samples were obtained after s torage for at least 7 days at room temperature. Conclusions: Mouthwash can be the method of choice to study gene polymorphi sms in periodontitis and other chronic inflammatory diseases.