Thromboxane A(2) release in ischemia and reperfusion of free flaps in rats, studied by microdialysis

Several studies have implicated enhanced eicosanoid production in reperfusi on injury. The reported study investigated the use of microdialysis in the in vivo measurement of thromboxane levels during reperfusion in ischemic an d reperfused experimental free muscle flaps. Microdialysis probes were inse rted, via a guide, into the gracilis and semitendinosus free flap in the ra t. The probe was perfused at a flow of 5 mul/min, with samples collected at intervals of 20 min, and analyzed by the ELISA technique. Animals were ran domly distributed into three groups. After ischemic periods of 2, 4, and 6 hr, respectively, the free muscle flaps were revascularized on the contrala teral femoral vessels. The mean thromboxane level during ischemia was 1785. 34 +/- 124.81 pg/ml. The mean levels of thromboxane rose significantly (p < 0.05), compared to base level, with 151.65 percent in the 2-hr ischemia gr oup, 192.33 percent in the 4-hr ischemia group, and 294.69 percent in the 6 -hr ischemia group, and correlated well with histologic observations. The r esults suggest that a microdialysis technique, combined with a sensitive as say for measuring thromboxane, is a useful method for in vivo monitoring of inflammatory processes during ischemia and reperfusion. The evolution of t hromboxane release following 6 hr of ischemia indicates that this mediator may be involved in facilitation of cell death, following ischemia and reper fusion, since its tissue level correlates with histologic tissue damage.