Isolation of Encephalitozoon cuniculi using primary tissue culture techniques from a rabbit in a colony showing encephalitozoonosis

Authors
Furuya, K Fukui, D Yamaguchi, M Nakaoka, Y Bando, G Kosuge, M
Citation
K. Furuya et al., Isolation of Encephalitozoon cuniculi using primary tissue culture techniques from a rabbit in a colony showing encephalitozoonosis, J VET MED S, 63(2), 2001, pp. 203-206
Citations number
15
Categorie Soggetti
Veterinary Medicine/Animal Health
Journal title
JOURNAL OF VETERINARY MEDICAL SCIENCE
ISSN journal
09167250 → ACNP
Volume
63
Issue
2
Year of publication
2001
Pages
203 - 206
Database
ISI
SICI code
0916-7250(200102)63:2<203:IOECUP>2.0.ZU;2-U
Abstract
Encephalitozoon spores were isolated in a primary tissue culture of the kid neys from an encephalitozoonosis-suspected rabbit in a municipal zoo in Hok kaido. The isolated spores were morphologically characteristic of microspor idial ones in chromotrope stain, and proven to be E. cuniculi by a polymera se chain reaction (PCR) with a species-specific primer set and by direct DN A sequencing of the PCR products.