Isolation of monoclonal antibodies that inhibit the binding of infectious bursal disease virus to LSCC-BK3 cells

Three hybridoma cell lines producing monoclonal antibodies (MAbs) against L SCC-BK3 cells which are susceptible to infectious bursal disease virus (IBD V) infection were produced and characterized. The MAbs, designated T7, Q11 and Q13, inhibited the attachment of IBDV to LSCC-BK3 cells. Furthermore, t hese MAbs bound to LSCC-BK3 but not to nonpermissive cells in flow cytometr y. MAb T7 detected a 110-kDa membrane protein of LSCC-BK3 cells, whereas Q1 1 and Q13 reacted with membrane proteins of molecular weights 58-, 85-, 90- and 110-kDa. These observations imply that the IlO-kDa protein recognized by all the MAbs is associated with IBDV binding. The MAbs established in th is study are useful for studying the interaction between IBDV and its targe t cell.