P. Ceccaroli et al., Effects of different carboyhydrate sources on the growth of Tuber borchii Vittad. mycelium strains in pure culture, MOL C BIOCH, 218(1-2), 2001, pp. 65-70
The influence of carbohydrate utilisation on the growth of three strains of
Vittad. mycelium (1BO, 17BO and 10RA) in culture was assessed using cultur
e media containing glucose (control), mannose or mannitol. Mannose was the
best substrate for growth of the strains and this was particularly evident
for strain 17BO. Mannitol instead was metabolized only by 10RA and 1BO. In
order to explain the different growth trends, analyses of enzyme levels, ki
netic parameters, protein patterns and the morphology of the three strains
were carried out. Our results show that these strains of mycelium were affe
cted by the substrates used in the media. The aim of the present work was t
o optimise the in vitro production of T. borchii mycelium for use in experi
ments which require the fungus in precise and reproducible conditions, such
as mycorrhizal synthesis or protein and nucleic acid extractions.