Construction of peptides mimicking a HIV-1 gp41 epitope recognized by virus-neutralizing antibodies 2F5

Authors
Tumanova, OY Kuvshinov, VN Azaev, MS Masharsky, AE Klimov, NA Kozlov, AP Ilyichev, AA Sandakhchiev, LS
Citation
Oy. Tumanova et al., Construction of peptides mimicking a HIV-1 gp41 epitope recognized by virus-neutralizing antibodies 2F5, MOL BIOL, 35(1), 2001, pp. 130-133
Citations number
19
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MOLECULAR BIOLOGY
ISSN journal
00268933 → ACNP
Volume
35
Issue
1
Year of publication
2001
Pages
130 - 133
Database
ISI
SICI code
0026-8933(200101/02)35:1<130:COPMAH>2.0.ZU;2-U
Abstract
A phage peptide library was screened with virus-neutralizing monoclonal ant ibodies (MAb) 2F5 which recognize a conserved epitope of HIV-1 gp41. Phages that expose peptides specifically binding with MAb 2F5 were selected by EL ISA. Amino acid sequence analysis revealed homology to region 662-671 of HI V-1 HB10 gp160 for most peptides. The major role in recognition was ascribe d to Asp-664, Lys-665, and Trp-666. The epitope-mimicking peptides were tes ted for immunogenicity. Antibodies to gp41 were detected in the serum of im munized rabbits.