S. Banniza et Ma. Rutherford, Diversity of isolates of Rhizoctonia solani AG-1 1A and their relationshipto other anastomosis groups based on pectic zymograms and molecular analysis, MYCOL RES, 105, 2001, pp. 33-40
Reference strains of Rhizoctonia solani from AG-1 1A, -1-1B, 1-1C, 2-1, 2-2
, and 3 to 9, isolates of AG-1 1A from various rice growing countries and f
ield isolates from a paddy rice field experiment in Cote d'Ivoire were anal
ysed by pectin enzyme analysis. A subset of these isolates was also analyse
d by A+T-rich DNA (AT-DNA) RFLPs, both with the objectives to study the div
ersity at various taxonomic levels and to obtain a clearer picture of the r
elationships between these isolates. Field isolates were submitted to simpl
e sequence repeat PCR (SSR-PCR). Pectin enzyme analysis as well as AT-DNA R
FLP showed that isolates of different anastomosis groups generated very dis
tinct patterns. Isolates of AG-1 1A from various countries had a lower degr
ee of variability in zymogram patterns and AT-DNA RFLPS, while zymograms an
d AT-DNA RFLPs of field isolates obtained from one field experiment consist
ed of one identical pattern. However, these isolates showed variation in RF
LPs obtained through SSR-PCR, indicating that they were not clones sensu st
ricto.