CHROMOSOMAL LOCATION OF GENES FOR HYDROXAMIC ACID ACCUMULATION IN TRITICUM-AESTIVUM L (WHEAT) USING WHEAT ANEUPLOIDS AND WHEAT SUBSTITUTIONLINES

Hydroxamic acids are defensive secondary metabolites in wheat, the mos t abundant aglucones being DIMBOA and DIBOA, The location of genes cod ing for the accumulation of hydroxamic acids by wheat (Triticum aestiv um L.) seedlings was explored comparing the euploid of cultivar Chines e Spring with aneuploids of the same cultivar, and substitution lines of cultivar Chinese Spring with cultivar Cheyenne. Significant differe nces in DIBOA and DIMBOA concentrations in young seedlings with respec t to the euploid (0.08 and 5.9 mmol kg(-1) fresh weight, respectively) were found in the following lines: monosomic lines 4A (3.9 and 2.8) a nd 5B (0.29 and 2.7), substitution line 4A (1.7 and 3,2) and nulli/tet ra lines N4AT4B (0.39 and 7.0), N4AT4D (0.20 and 0.48), N5BT5A (0.12 a nd 0.34) and N5BT5D (0.06 and 0.63). These results suggest the involve ment of chromosomes of group 4 and also chromosome 5B in the accumulat ion of hydroxamic acids in wheat seedlings.