Induction of a tyrosinase mRNA in Agaricus bisporus upon treatment with a tolaasin preparation from Pseudomonas tolaasii

Authors
Soler-Rivas, C Moller, AC Arpin, N Olivier, JM Wichers, HJ
Citation
C. Soler-rivas et al., Induction of a tyrosinase mRNA in Agaricus bisporus upon treatment with a tolaasin preparation from Pseudomonas tolaasii, PHYSL MOL P, 58(2), 2001, pp. 95-99
Citations number
27
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
ISSN journal
08855765 → ACNP
Volume
58
Issue
2
Year of publication
2001
Pages
95 - 99
Database
ISI
SICI code
0885-5765(200102)58:2<95:IOATMI>2.0.ZU;2-A
Abstract
In Agaricus bisporus infected with Pseudomonas tolaasii, or treated with a partially purified tolaasin extract or with purified tolaasin. induction of a specific mRNA of the two sequences encoding tyrosinase was found. Tyrosi nase isoforms in samples treated with several concentrations of a partially purified tolaasin (PT) extract were visualized after Western analysis and staining with polyclonal tyrosinase-specific antibodies. Similar concentrat ion of a 67 kDa latent tyrosinase was shown in all the samples including th e water-treated control hut the treatment with the PT extract caused the po st-translational modification of the protein into an active tyrosinase of 4 3 kDa. (C) 2001 Academic Press.