Basic characterization of two barley hemA promoter regions reveals stem-loop structures and suggests a regulatory role in Poaceae tetrapyrrole biosynthesis
M. Hansson, Basic characterization of two barley hemA promoter regions reveals stem-loop structures and suggests a regulatory role in Poaceae tetrapyrrole biosynthesis, PL PHYS BIO, 39(2), 2001, pp. 155-160
5-Aminolevulinic acid (ALA) is a common biosynthetic intermediate of both c
hlorophyll and heme. Glutamyl tRNA(Glu) reductase, encoded by the hemA gene
, is the initial enzyme of ALA biosynthesis. The presence of several hemA g
enes provides a possible explanation to the ability of plants to meet the d
ifferent requirement of chlorophyll and heme using a common biosynthetic pa
thway. In this work, two barley (Hordeum vulgare L.) hemA genes were cloned
from chromosomal DNA. The hemA1 and hemA2 genes are located at the long an
d short arm, respectively, of barley chromosome 5 (1H). Although having 98
% identical coding sequences the promoter regions of hemA1 and hemA2 are ve
ry different, which could explain their different expression patterns. The
first 170 nucleotides of the coding region of both genes contain several in
verted repeat regions capable of forming stern-loop structures, which could
be involved in the regulation of tetrapyrrole biosynthesis in species belo
nging to the Poaceae family. (C) 2001 Editions scientifiques et medicales E
lsevier SAS.