A structured model for intracellular EcoRI endonuclease production by recombinant E. coli 294

A three-compartment structured model is proposed for simulating batch culti vation of growth, substrate utilization and intracellular EcoRI endonucleas e production by E. coli 294, an overproducing recombinant strain containing plasmid pPG430. Model development and verification are based on the dynami c changes in biomass formation and production of intracellular components s uch as total protein, total RNA, plasmid DNA and EcoRI endonuclease in supp lemented minimal media with glucose as the carbon source. The three compart ment model presented here has potential for evolutionary modelling work bec ause of its simplicity and can easily be applied to other recombinant micro organisms and to industrial fermentations using complex media. (C) 2001 Pub lished by Elsevier Science Ltd. All rights reserved.