Ultra-fast gradient vs. fast isocratic chromatography in bioanalytical quantification by liquid chromatography/tandem mass spectrometry

Liquid chromatagraphy/tandem mass spectrometry (LC/MS/MS) methods developed for quantification using rapid ('ballistic') gradients on narrow bore, sho rt HPLC columns have been previously described by this laboratory. This pap er compares the fast gradient approach with the more traditional high-organ ic isocratic LC/MS/MS methods. The comparison is based on an analysis of th e effectiveness of the chromatographic separations when using the two appro aches (i.e. k' N, and W). The data presented herein are derived from actual biological samples analyzed as part of the drug discovery process. Copyrig ht (C) 2001 John Wiley & Sons, Ltd.