AN AMPEROMETRIC ENZYME ELECTRODE FOR GLUCOSE USING IMMOBILIZED GLUCOSE-OXIDASE IN A FERROCENE ATTACHED POLY(4-VINYLPYRIDINE) MULTILAYER FILM

An amperometric enzyme electrode for glucose has been prepared by immo bilized glucose oxidase (GOD) in a multilayer film based on a thiol se lf-assembled monolayer at a gold electrode. The multilayer film of GOD and ferrocene attached poly(4-vinylpyridine) (Fc-PVP) complex is form ed by electrostatic force. Fc-PVP as a polyelectrolyte in which ferroc ene is as a mediator, acts with GOD to form a complex. The linear rang e of the enzyme electrode is extended up to 10mmol/L of glucose due to the addition of 5 GOD layers. The enzyme electrode is sensitive to gl ucose, 0.10 mmol/L glucose leads to a response current of 22nA and the detection limit is down to 0.01 mmol/L. Ascorbic acid and uric acid h ave no interference for the determination of glucose. The enzyme elect rode is stable over 3 weeks.