NADP(+) - BASED ENZYME ASSAY FOR D-ISOCITRATE

Authors
SHARMA A QUANTRILL NSM ROGERS KR
Citation
A. Sharma et al., NADP(+) - BASED ENZYME ASSAY FOR D-ISOCITRATE, Analytical letters, 30(9), 1997, pp. 1667-1674
Citations number
6
Categorie Soggetti
Chemistry Analytical
Journal title
Analytical letters → ACNP
ISSN journal
00032719
Volume
30
Issue
9
Year of publication
1997
Pages
1667 - 1674
Database
ISI
SICI code
0003-2719(1997)30:9<1667:N-BEAF>2.0.ZU;2-U
Abstract
A novel procedure for the measurement of D-isocitrate is described tha t utilizes the fluorescence quenching of thionine by the reduced form of the cofactor NADPH, which results from the isocitrate dehydrogenase -catalyzed conversion of D-isocitrate to 2-oxoglutarate. The procedure is termed substrate induced-quenching (SIQ) assay and results in a fl uorescence-quenching constant (K-SIQ) of 4443 M-1 obtained from data c ollected over the range of 0-500 mu M isocitrate.