Study of different hormone-sensitive lipase concentrations using a surfaceplasmon resonance sensor

This paper presents a series of experimental results including those based on the surface plasmon resonance (SPR) to investigate the parameters for de termining the concentration and form of various hormone sensitive lipase (H SL) solutions. HSL is a rate-limiting enzyme for the metabolism of stored f at. This small protein was not previously known to possess optical properti es permitting studies by attenuated total internal reflection (ATR) spectro scopy or by the SPR method. Therefore, basic physical measurements were nee ded. The HSL solutions are typically contained in different buffers that ch emically affect the metal layers in the SPR experiments and cause problems with film integrity. Different experiments employing different metal layers were conducted. The results were compared with those of the ATR method, sh owing the expected greater sensitivity of the surface plasmon resonance met hod. It was determined that the protein solutions may be usefully examined by both methods ultimately permitting measurement of oligomer formation as a function of concentration in future work. (C) 2001 Elsevier Science B.V. All rights reserved.