Seeding of nitrifying bacteria into the activated sludge process was studie
d both theoretically and experimentally. A simple model was developed for p
rediction of the effects of seeding of nitrifying bacteria from a separate
stage into the activated sludge process. The purpose of seeding is to impro
ve the treatment results and the process stability as well as to decrease t
he volume requirements of the process. Pilot plant studies were carried out
at the Uppsala municipal wastewater treatment plant in order to evaluate t
he effects of seeding. One line was supplied with supernatant from dewateri
ng of digested sludge and the nitrification process gave an activated sludg
e with a high fraction of nitrifying bacteria, suitable for seeding. The ot
her line was supplied with pre-precipitated wastewater and with the excess
sludge from the line treating the supernatant. The experimental results sho
wed that nitrification could be obtained at sludge ages that would otherwis
e preclude nitrification. Performance relationships for the system develope
d, based on laboratory and on-line measurements were studied and are presen
ted. The studies show that seeding may decrease the necessary volume needs
for a stable nitrification process and that the effects could be predicted
by use of a simple model.