ASCUS, mature metaplastic type - Cytologic diagnosis and follow-up

OBJECTIVE: To study the cytologic criteria for follow-up of mature metaplas tic cells within the atypical squamous cells of undetermined significance ( ASCUS) category STUDY DESIGN: Squamous epithelial abnormalities between January 1994 and Ju ne 1997 at our institution totaled 2,632 and included squamous carcinoma (1 ), high grade squamous intraepithelial lesions (278), low grade squamous in traepithelial lesions (875) and ASCUS (1,478). From the ASCUS group, 134 (9 .06%) were metaplastic; 89 were selected for reaction. Criteria for case se lection were follow-up with tissue biopsy or at least two Pap smears and no previous epithelial abnormality. Patients ranged from 27 to 70 years of ag e. Parameters tabulated included number of abnormal cells per slide, their architecture, cell size, shape, cytoplasmic hire and texture, nuclear size and contour, chromatin pattern and nucleoli. Additionally, specimens were r eviewed for hormonal status and inflamnation. The findings were correlated with follow-up data. RESULTS: Cells generally appeared single or in loose, monolayered sheets of three to seven cells per group. The cells were well demarcated, polygonal or oval and ranged from 11 to 30 mum with cyanophilic or eosinophilic thick ened cytoplasm. The round to oval nuclei with slight ir regularity showed a minimally increased nuclear/cytoplasmic untie with stippled chromatin. Upo n review, 69 smears were confirmed as ASCUS-M. Follow-up revealed 42 with b enign findings 9 with persistent ASCUS/ASCUS-M and 18 with low grade squamo us intraepithelial lesions.