An electrophoretic analysis of the seed protein body proteins from Pinus nigra

Protein bodies (PBs) of European black pine (Pirus nigra Am.) were isolated from mature seeds. Extracted soluble matrix proteins and crystalloid prote ins PBs proteins were investigated by SDS-PAGE electrophoresis in presence and absence of 2-mercaptoethanol. The proteins of molecular masses 16, 17, 18, 61 and 65 kDa were presented only in crystalloid protein samples. Only 15 kDa protein was present in soluble matrix proteins and not in crystalloi d proteins. Another protein bands were present in both soluble matrix and c rystalloid proteins. 20, 37, 38, 39 and 48 kDa proteins were strongly visib le among crystalloid proteins. Bands of 23 and 32 kDa were more visible in soluble matrix protein samples. Different composition in crystalloid protei ns was found in absence of 2-mercaptoethanol: no proteins with molecular ma ss 71 kDa and more proteins in soluble matrix. In case of crystalloid prote ins we detected 7 protein bands in interval from 71 to 212 kDa.