Characterization of direct readout contacts of the Myb DNA-binding domain

Authors
Schurmann, A Sokolowski, R Haas, M Wolfes, H
Citation
A. Schurmann et al., Characterization of direct readout contacts of the Myb DNA-binding domain, BL CELL M D, 27(2), 2001, pp. 464-469
Citations number
23
Categorie Soggetti
Cardiovascular & Hematology Research
Journal title
BLOOD CELLS MOLECULES AND DISEASES
ISSN journal
10799796 → ACNP
Volume
27
Issue
2
Year of publication
2001
Pages
464 - 469
Database
ISI
SICI code
1079-9796(200103)27:2<464:CODRCO>2.0.ZU;2-G
Abstract
The Myb protein contacts its recognition sequence by means of direct protei n-DNA interactions. We used site-directed mutagenesis in order to substitut e amino acids crucial for these contacts and probed the mutant proteins for their DNA-binding and transactiving activities. We could show that amino a cids involved in direct readout contacts do not contribute equivalently in the recognition process. (C) 2001 Academic Press.