Jp. Mathis et al., Carboxyl terminal peptides derived from prepro-orphanin FQ/nociceptin (ppOFQ/N) are produced in the hypothalamus and possess analgesic bioactivities, BRAIN RES, 895(1-2), 2001, pp. 89-94
Orphanin FQ/nociceptin (OFQ/N), the endogenous ligand for the ORL-1/KOR-3 r
eceptor, produces a wide variety of behavioral responses. Its precursor pro
tein, prepro-OFQ/N (ppOFQ/N) contains several series of amino acids bounded
by pairs of basic amino acids. raising the possibility that additional fun
ctional neuropeptides could be generated by proteolytic posttranslational p
rocessing. Several of these processing products have been shown to have pha
rmacological activity, including the 17 amino acid peptide OFQ/N (mppOFQ/N1
40-157) which is a major product of this precursor in the hypothalamus. Her
e wt: have used a newly developed radioimmunoassay and RP-HPLC to detect mp
pOFQ/N160-187 in mouse hypothalamic extracts, Murine ppOFQ/N160-187 has pot
ent analgesic activity supraspinally (3.3 nmol, i.c.v,) and spinally (4.3 n
mol, i.t.). This analgesic activity was reversed by the opioid antagonist n
aloxone (5 mg/kg, s.c.) and kappa(1)-selective opioid antagonist nor-BNI (6
0 mug. i.c.v.), despite the inability of ppOFQ/N160-187 to compete binding
in mu, delta, kappa(1), kappa(3). or OFQ/N binding assays. These findings s
uggest that murine ppOFQ/N160-187 may be a physiologically relevant neurope
ptide with a novel mechanism of action. (C) 2001 Elsevier Science B.V. All
rights reserved.