Citation
K. Knight et al., Immobilization of lipase from Fusarium solani FS1, BRAZ J MICR, 31(3), 2000, pp. 220-222
Citations number
11
Categorie Soggetti
Biology
Journal title
BRAZILIAN JOURNAL OF MICROBIOLOGY
ISSN journal
15178382
→ ACNP
Volume
31
Issue
3
Year of publication
2000
Pages
220 - 222
Database
ISI
SICI code
1517-8382(200007/09)31:3<220:IOLFFS>2.0.ZU;2-#
Abstract
Lipase from Fusarium solani FSl was immobilized by covalent attachment to p
olyacrylamide beads and onto magnetized Dacron, retaining 12% and 97% of ac
tivity, respectively. Lipase was also entrapped within polyacrylamide beads
, retaining 53% of activity. Investigations of the kinetic characteristics
of the immobilized derivatives using triolein as substrate showed that lipa
se immobilized onto polyacrilamide beads and Dacron did not follow Michaeli
s-Menten kinetics.