P. Cordoba et al., Evaluation of antibodies against a rubella virus neutralizing domain for determination of immune status, CL DIAG LAB, 7(6), 2000, pp. 964-966
The protective immune responses against rubella virus (RV) are related to i
ts neutralizing epitopes, an issue that is important to consider when asses
sing the immune status of patients with remote infection, In the present pa
per, we compare the antibodies detected by a synthetic-peptide-based enzyme
immunoassay (EIA) with antibodies detected by the traditional technique of
hemagglutination inhibition (HIA) in patients with remote RV infection. Th
e synthetic peptide used as an antigen (SP15) represents a neutralizing epi
tope that corresponds to amino acids 208 to 239 of the E1 glycoprotein. The
SP15-EIA was developed, all variables that affected the assay were standar
dized, and the test was validated using reference sera. Serum samples (n =
129) from patients with remote RV infection were tested by HIA and SP15-EIA
. Discrepant sera were assayed by MEIA (IMX/Abbot). The comparison between
HIA and SP15-EIA, taking HIA as the standard methodology for determining im
mune status, showed that SP15-EIA is very specific and sensitive for detect
ing protecting antibodies (specificity, 100%; sensitivity, 98.20%). This st
udy demonstrates that antibodies against the neutralizing domain represente
d by SP15 would be important in the memory response after natural infection
and may be a good tool in the determination of the true immune status of p
atients with remote infection with regard to RV.