Rapid detection of Salmonella enterica serovar choleraesuis in blood cultures by a dot blot enzyme-linked immunosorbent assay

A dot blot enzyme-linked immunosorbent assay (ELISA) with a monoclonal anti body specific to phase1-c Salmonella was developed for the direct detection of Salmonella enterica serovar Choleraesuis in blood cultures. This system was applied to the identification of serovar Choleraesuis, and the results were compared with those obtained by a conventional biochemical method. It was revealed that all 12 samples identified to be infected with serovar Ch oleraesuis were positive on testing by the ELISA. In contrast, 77 samples i nfected with bacteria commonly isolated from the blood were not reactive by the ELISA. The calculated sensitivity and specificity of the established a ssay are 100%.