Monoclonal antibody binding to a surface-exposed epitope on Cowdria ruminantium that is conserved among eight strains

Authors
Shompole, S Rurangirwa, FR Wambugu, A Sitienei, J Mwangi, DM Musoke, AJ Mahan, S Wells, CW McGuire, TC
Citation
S. Shompole et al., Monoclonal antibody binding to a surface-exposed epitope on Cowdria ruminantium that is conserved among eight strains, CL DIAG LAB, 7(6), 2000, pp. 983-986
Citations number
33
Categorie Soggetti
Immunology
Journal title
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
ISSN journal
1071412X → ACNP
Volume
7
Issue
6
Year of publication
2000
Pages
983 - 986
Database
ISI
SICI code
1071-412X(200011)7:6<983:MABTAS>2.0.ZU;2-1
Abstract
Monoclonal antibodies (MAb) binding to Cowdria ruminantium elementary bodie s (EB) were identified by enzyme-linked immunosorbent assay, and surface bi nding of one MAb (446.15) to intact EB was determined by immunofluorescence , immunogold labeling, and transmission electron microscopy. MAb 346.15 bou nd an antigen of approximately 43 kDa in immunoblots of eight geographicall y distinct strains. The MAb did not react with Ehrlichia canis antigens or uninfected bovine endothelial cell lysate and may be useful in diagnostic a ssays and vaccine development.