SUPPRESSION OF HTV-1 TRANSCRIPTION BY BETA-CHEMOKINES RANTES, MIP1-ALPHA, AND MIP-1-BETA IS NOT MEDIATED BY THE NFAT-1 ENHANCER ELEMENT

Soluble factors derived from human CD8+ T-lymphocytes inhibit HIV-I re plication by suppressing transcription from the viral long terminal re peat (LTR), an effect shown to be mediated in part by an NFAT-1 enhanc er sequence, We show here that the CD8+ derived beta-chemokines, RANTE S, MIP1-alpha, and MIP-1 beta, known suppressors of HIV-I replication in human peripheral blood mononuclear cells, can suppress transcriptio n from the HIV-1 LTR in transient transfection assays in cells of the Jurkat (acute T leukemia) line, Surprisingly, the suppression mediated by these beta-chemokines persisted in the absence of an intact NFAT-1 element, suggesting that there are at least two classes of HIV-1 supp ressor factors - NFAT-1-dependent and NFAT-1-independent factors - pro duced by CD8+ T-lymphocytes. (C) 1997 Federation of European Biochemic al Societies.