J. Marquardt et al., ISOLATION AND CHARACTERIZATION OF BILIPROTEIN AGGREGATES FROM ACARYOCHLORIS-MARINA, A PROCHLORON-LIKE PROKARYOTE CONTAINING MAINLY CHLOROPHYLL-D, FEBS letters, 410(2-3), 1997, pp. 428-432
Phycobiliprotein aggregates were isolated from the prokaryote Acaryoch
loris marina, containing chlorophyll d as major pigment. In the electr
on microscope the biliprotein aggregates appear as rod-shaped structur
es of 26.0 x 11.3 nm, composed of four ring-shaped subunits 5.8 nm thi
ck and 11.7 nm in diameter. Spectral data indicate that the aggregates
contain two types of biliproteins: phycocyanin and an allophycocyanin
-type pigment, with very efficient energy transfer from the phycocyani
n- to allophycocyanin-type constituent. The chromophore-binding polype
ptides of the pigments have apparent molecular masses of 16.2 and 17.4
kDa, They crossreact,vith antibodies against phycocyanin and allophyc
ocyanin from a red alga. (C) 1997 Federation of European Biochemical S
ocieties.